2D4P

Crystal structure of TTHA1254 (wild type) from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-02-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	61.721, 61.721, 74.707
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.16958
Rwork	0.168
R-free	0.19485
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d4o
RMSD bond length	0.013
RMSD bond angle	1.428
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.700
Number of reflections	18129

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG3350, Tris, NaCl, Ammonium nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 277K