2D4O
Crystal structure of TTHA1254 (I68M mutant) from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-30 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.96300, 0.97896, 0.97932 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 58.463, 58.463, 77.679 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.18305 |
| Rwork | 0.180 |
| R-free | 0.23574 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.487 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 14538 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG3350, Hepes, NaCl, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
