2D4J
Transformed monoclinic crystal of hen egg-white lysozyme from a heavy water solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 290 |
| Detector technology | CCD |
| Collection date | 2004-07-30 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.460, 58.180, 30.850 |
| Unit cell angles | 90.00, 111.33, 90.00 |
Refinement procedure
| Resolution | 12.300 - 1.160 |
| R-factor | 0.098 |
| Rwork | 0.097 |
| R-free | 0.13300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xek |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.027 |
| Data reduction software | SaintPLus |
| Data scaling software | SAINTPLUS |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.300 | 1.200 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.106 | 0.299 |
| Number of reflections | 25242 | |
| Completeness [%] | 83.6 | 52.8 |
| Redundancy | 3.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | phase transition | 4.5 | 293 | The crystal was created by the dehydration-induced phase transition of a monoclinic crystal obtained from a heavy water solution at pD 4.5, temperature 293K |
