2D4G

Structure of YjcG protein, a putative 2'-5' RNA ligase from Bacillus subtilis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-30
Detector	MARRESEARCH
Wavelength(s)	0.981, 0.97, 1.2
Spacegroup name	C 1 2 1
Unit cell lengths	99.660, 73.930, 61.770
Unit cell angles	90.00, 113.56, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.235
Rwork	0.224
R-free	0.26400
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.03)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.079	0.157
Number of reflections	33260
Completeness [%]	98.6	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	0.1M Bis-tris, 24% PEG-MME2000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	0.1M Bis-tris, 24% PEG-MME2000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	0.1M Bis-tris, 24% PEG-MME2000, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K