2D4C
Crystal structure of the endophilin BAR domain mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-06 |
| Detector | RIGAKU |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.344, 212.217, 54.317 |
| Unit cell angles | 90.00, 96.97, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x03 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.959 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.098 | 0.323 |
| Number of reflections | 38763 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.6 | 93.5 |
| Redundancy | 3.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 100mM HEPES, 200mM calcium acetate, 10mM DTT, 20% PEG3350, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
