2D47
MOLECULAR STRUCTURE OF A COMPLETE TURN OF A-DNA
Experimental procedure
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Detector | ENRAF-NONIUS CAD4 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.200, 45.200, 65.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.000 |
| R-factor | 0.177 |
| Refinement software | NUCLSQ ((MODIFIED BY G.J.QUIGLEY)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.039 * |
| Number of reflections | 2333 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | pH 7.00, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | CACL2 | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dodecamer | 1 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 30 (mM) | |
| 3 | 1 | drop | calcium chloride | 5 (mM) | |
| 4 | 1 | drop | spermine tetrachloride | 1.25 (mM) | |
| 5 | 1 | drop | MPD | 5 (%(v/v)) | |
| 6 | 1 | reservoir | MPD | 20 (%) |
