2D3L
Crystal structure of maltohexaose-producing amylase from Bacillus sp.707 complexed with maltopentaose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.450, 82.460, 126.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.300 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Native structure (1WP6) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.130 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.168 | 0.346 |
| Number of reflections | 31896 | |
| Completeness [%] | 97.8 | 86.7 |
| Redundancy | 2.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2,4-dimethylpentanediol, Tris-HCl, Ammonium Phosphate, Calcium chloride, Sodium chloride, Maltopentaose, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
