2D3F
Crystal structures of collagen model peptides (Pro-Pro-Gly)4-Pro-Hyp-Gly-(Pro-Pro-Gly)4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.082, 26.532, 79.843 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 2CUO |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.022 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.530 | 1.310 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.091 | 0.331 |
| Number of reflections | 26765 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 89.3 | 93.7 |
| Redundancy | 6.87 | 7.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
