2D3E
Crystal structure of the C-Terminal fragment of rabbit skeletal alpha-tropomyosin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-04-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.080, 46.860, 95.260 |
| Unit cell angles | 101.49, 101.66, 90.13 |
Refinement procedure
| Resolution | 45.670 - 2.600 |
| R-factor | 0.254 |
| Rwork | 0.249 |
| R-free | 0.30900 |
| Structure solution method | MR |
| Starting model (for MR) | PDB CODE 1IC2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | CRYSTAL (CLEAR) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.670 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 | 0.184 |
| Number of reflections | 19366 | |
| <I/σ(I)> | 29 | 9 |
| Completeness [%] | 97.4 | 97.4 |
| Redundancy | 16.56 | 17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | MPD, AMMONIUM ACETATE, SODIUM CITRATE, pH 5.20, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
