2D3C
Crystal Structure of the Maize Glutamine Synthetase complexed with ADP and Phosphinothricin Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-05 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.958, 191.012, 118.124 |
| Unit cell angles | 90.00, 101.23, 90.00 |
Refinement procedure
| Resolution | 27.360 - 3.810 |
| R-factor | 0.18694 |
| Rwork | 0.185 |
| R-free | 0.22943 |
| Structure solution method | MIR |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.763 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.145 | 0.355 |
| Number of reflections | 34283 | |
| <I/σ(I)> | 5.8 | 1.8 |
| Completeness [%] | 82.5 | 77.1 |
| Redundancy | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 9% PEG8000, 5% MPD, 100MM Tris-HCl, 10MM MnCl2, 1MM ATP, 1MM phosphinothricin, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
