2D2G
OpdA from Agrobacterium radiobacter with bound product dimethylthiophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-10 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5148 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.956, 108.956, 62.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.850 |
| R-factor | 0.16742 |
| Rwork | 0.166 |
| R-free | 0.19367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 35872 | |
| Completeness [%] | 97.7 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 20% PEG 3350, 0.2M NaNO3, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
