2D2F
Crystal structure of atypical cytoplasmic ABC-ATPase SufC from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Detector technology | CCD |
| Collection date | 2004-03-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.336, 71.864, 49.776 |
| Unit cell angles | 90.00, 105.71, 90.00 |
Refinement procedure
| Resolution | 39.870 - 1.900 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 17807 | |
| <I/σ(I)> | 22.7 | 5.4 |
| Completeness [%] | 99.5 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 293 | 0.1M Tris, 15% PEG 4000, 150mM MgCl2, 20% glycerol, pH 8.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
