2D1L
Structure of F-actin binding domain IMD of MIM (Missing In Metastasis)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97908, 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.503, 37.319, 129.016 |
| Unit cell angles | 90.00, 94.07, 90.00 |
Refinement procedure
| Resolution | 48.100 - 1.850 |
| R-factor | 0.18577 |
| Rwork | 0.183 |
| R-free | 0.22829 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.100 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.057 | 0.380 |
| Number of reflections | 43473 | |
| <I/σ(I)> | 30.4 | 3.6 |
| Completeness [%] | 98.7 | 88.4 |
| Redundancy | 10.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | lithium chloride, PEG2000 MME, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
