2D1I
Structure of human Atg4b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-09 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.278, 161.321, 51.271 |
Unit cell angles | 90.00, 119.58, 90.00 |
Refinement procedure
Resolution | 44.590 - 2.000 |
R-factor | 0.22351 |
Rwork | 0.220 |
R-free | 0.28317 |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 1.422 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.590 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 48166 | |
Completeness [%] | 98.7 | 99.1 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.65M tri-Sodium citrate dihydrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |