2D1G

Structure of Francisella tularensis Acid Phosphatase A (AcpA) bound to orthovanadate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	160
Detector technology	CCD
Collection date	2004-07-01
Detector	ADSC QUANTUM 210
Wavelength(s)	1.127129
Spacegroup name	C 2 2 21
Unit cell lengths	112.081, 144.399, 123.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.280 - 1.750
Rwork	0.198
R-free	0.23100
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.683
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.047	0.357
Number of reflections	98137
Completeness [%]	97.2
Redundancy	4.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	PEG 1500, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	6	298	PEG 1500, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	6	298	PEG 1500, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 298K