2D0D
Crystal Structure of a Meta-cleavage Product Hydrolase (CumD) A129V Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.650, 116.623, 78.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.840 - 1.650 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.19100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1iup |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.840 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 42732 | |
| <I/σ(I)> | 26 | 5.5 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 298 | PEG 3000, sodium phosphate/citrate, sodium chloride, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
