2D00

Subunit F of V-type ATPase/synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-03
Detector	MARRESEARCH
Wavelength(s)	0.9790
Spacegroup name	P 21 21 2
Unit cell lengths	81.705, 138.295, 66.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.200
Rwork	0.214
R-free	0.26221
Structure solution method	MAD
RMSD bond length	0.033
RMSD bond angle	2.492
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	38602
Completeness [%]	99.6	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PEG 400, calcium acetate, potassium chloride, MES, MOPS, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PEG 400, calcium acetate, potassium chloride, MES, MOPS, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PEG 400, calcium acetate, potassium chloride, MES, MOPS, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K