2CZT
lipocalin-type prostaglandin D synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.01 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.300, 67.100, 104.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.245 |
| Rwork | 0.242 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.385 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | |
| High resolution limit [Å] | 2.000 | |
| Number of reflections | 11368 | |
| Completeness [%] | 77.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295.5 | sodium citrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K |
