2CZ3

Crystal structure of glutathione transferase zeta 1-1 (maleylacetoacetate isomerase) from Mus musculus (form-2 crystal)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-04-04
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	55.812, 55.812, 214.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.710 - 2.300
R-factor	0.2207
Rwork	0.218
R-free	0.27474
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.287
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	sodium acetate, sodium cacodylate, PEG8000, glycerol, Tris, sodium chloride, dithiothreitol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K