2CYF
The Crystal Structure of Canavalia Maritima Lectin (ConM) in Complex with Trehalose and Maltose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-05 |
Detector | MARRESEARCH |
Wavelength(s) | 1.43 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 67.033, 97.332, 71.086 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 9.980 - 1.800 |
R-factor | 0.19355 |
Rwork | 0.186 |
R-free | 0.23802 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.869 |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9.990 | 1.910 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 50831 | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Hepes-Na, ammonium sulfate, PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |