2CXH
Crystal structure of probable ribosomal biogenesis protein from Aeropyrum pernix K1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.171, 47.364, 124.675 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.710 - 1.800 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.20900 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.796 | 1.760 |
| High resolution limit [Å] | 1.697 | 1.700 |
| Number of reflections | 22973 | |
| <I/σ(I)> | 23.616 | 2.5 |
| Completeness [%] | 93.6 | 95.4 |
| Redundancy | 8.36835 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.01M Tris-HCl pH 8.0, 0.15M NaCl, 0.001M DTT, 0.05M tri-sodium citrate pH 5.6, 10% v/v 2-propanol, 10% w/v polyethylene glycol 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
