2CXC

Crystal structure of archaeal transcription termination factor NusA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-03
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97885, 0.97920, 0.97300
Spacegroup name	I 41
Unit cell lengths	100.348, 100.348, 45.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.500 - 2.000
Rwork	0.184
R-free	0.21120
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	18141
<I/σ(I)>	35.83	2.9713
Completeness [%]	99.8	100
Redundancy	7.3521	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.8	293	PEG 3350, sodium acetate, pH 4.8, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	4.8	293	PEG 3350, sodium acetate, pH 4.8, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	4.8	293	PEG 3350, sodium acetate, pH 4.8, VAPOR DIFFUSION, temperature 293K