2CXB

CRYSTALLIZATION AND X-RAY STRUCTURE DETERMINATION OF CYTOCHROME C2 FROM RHODOBACTER SPHAEROIDES IN THREE CRYSTAL FORMS

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	45.310, 37.970, 37.600
Unit cell angles	102.94, 72.44, 90.57

Refinement procedure

Resolution	6.000 - 1.950
R-factor	0.172
RMSD bond length	0.008
RMSD bond angle	1.580
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.950
Rmerge	0.056 *
Number of reflections	14690
Completeness [%]	77.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	cytochrome c2	10 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	drop	PEG4000	5 (%(w/v))
4	1	reservoir	PEG4000	20 (%(w/v))