2CXA
Crystal structure of Leucyl/phenylalanyl-tRNA protein transferase from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9797, 0.9795, 0.9680 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 68.277, 44.784, 76.376 |
| Unit cell angles | 90.00, 108.36, 90.00 |
Refinement procedure
| Resolution | 36.840 - 1.600 |
| Rwork | 0.187 |
| R-free | 0.21700 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.860 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 28862 | |
| Completeness [%] | 98.7 | 93.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG3350, NaCl, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
