2CX3
Crystal structure of a bacterioferritin comigratory protein peroxiredoxin from the Aeropyrum pernix K1 (form-1 crystal)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-01 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.96400, 0.97909, 0.97942 |
| Spacegroup name | P 64 |
| Unit cell lengths | 126.990, 126.990, 104.856 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.330 - 2.640 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.640 |
| Number of reflections | 54115 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | ammonium sulfate, 2-propanol, Tris, sodium chloride, dithiothreitol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
