2CWN

Crystal structure of mouse transaldolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-26
Detector	MARRESEARCH
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	79.050, 108.009, 75.608
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.100
R-factor	0.196
Rwork	0.196
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f05
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	38360
<I/σ(I)>	15.1	3.3
Completeness [%]	99.0	93.8
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	293	Bis-Tris, PEG3350, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K