2CWM
Native Crystal Structure of NO releasing inductive lectin from seeds of the Canavalia maritima (ConM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.4270 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.916, 97.617, 71.021 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.400 - 1.950 |
| R-factor | 0.19149 |
| Rwork | 0.189 |
| R-free | 0.23692 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.052 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.910 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.122 | |
| Number of reflections | 34238 | |
| Completeness [%] | 97.9 | 97.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Hepes-Na, ammonium sulfate, PEG 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
