2CWD
Crystal Structure of TT1001 protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-15 |
| Detector | RIGAKU |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.108, 31.293, 102.605 |
| Unit cell angles | 90.00, 98.70, 90.00 |
Refinement procedure
| Resolution | 38.040 - 1.900 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dg9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.075 | |
| Number of reflections | 48903 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 295 | Magnesium acetate tetrahydrate, sodium cacodylate, PEG 8K, pH 6.5, microbatch, temperature 295K |
