2CVO

Crystal structure of putative N-acetyl-gamma-glutamyl-phosphate reductase (AK071544) from rice (Oryza sativa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-07
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0080
Spacegroup name	P 61
Unit cell lengths	86.112, 86.112, 316.333
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.930 - 2.200
Rwork	0.171
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vkn
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.042	0.064
Number of reflections	65873
<I/σ(I)>	50.8	23.7
Completeness [%]	97.2	88.8
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M Tris-HCl, 0.72M sodium formate, 22.5% PEG MME 2000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K