2CVH

Crystal structure of the RadB recombinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-01
Detector	ADSC QUAMTUM 4r
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	59.731, 82.867, 96.788
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.200
Rwork	0.193
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cvf
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.880	2.140
High resolution limit [Å]	2.030	2.030
Number of reflections	31513
Completeness [%]	99.8	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	20% PEG 6000, 2.0M lithium sulphate, 50mM citrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K