2CVC
Crystal structure of High-Molecular Weight Cytochrome c from Desulfovibrio vulgaris (Hildenborough)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 62 |
| Unit cell lengths | 108.670, 108.670, 103.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.350 - 2.000 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 4.400 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.561 |
| Number of reflections | 47050 | |
| <I/σ(I)> | 8.7 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 288 | PEG600, 2-morpholinoethanesulfonic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
