2CVB
Crystal structure of a thioredoxin-like protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-31 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.90000, 0.97919, 0.97942 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.420, 73.420, 96.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.250 - 1.800 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.180 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.051 | 0.188 |
| <I/σ(I)> | 26.9 | 10.1 |
| Completeness [%] | 95.1 | 97.3 |
| Redundancy | 9.54 | 9.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | 22% PEG3350, 0.3M diammonium hydrogen citrate, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
