2CV9
Crystal structure of a hypothetical protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-02 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9, 0.9791, 0.9795 |
| Spacegroup name | P 41 |
| Unit cell lengths | 76.481, 76.481, 193.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.780 - 2.500 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.660 |
| High resolution limit [Å] | 2.501 | 2.500 |
| Rmerge | 0.047 | 0.166 |
| Number of reflections | 75502 | |
| <I/σ(I)> | 33.1 | 9 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | magnesium chloride, Sodium Citrate, MPD, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
