2CV6
Crystal Structure of 8Salpha Globulin, the Major Seed Storage Protein of Mungbean
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 146.600, 146.600, 53.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.990 - 2.650 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | SADIE |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.710 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Number of reflections | 11640 | |
| Completeness [%] | 89.6 | 71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 1000, NaCl, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
