2CV1
Glutamyl-tRNA synthetase from Thermus thermophilus in complex with tRNA(Glu), ATP, and an analog of L-glutamate: a quaternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-04 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 110.624, 218.818, 134.858 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.360 - 2.410 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | |
| Number of reflections | 61084 | |
| <I/σ(I)> | 16.4 | 2.1 |
| Completeness [%] | 96.3 | 95.9 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | PEG1500, Mops-Na, ammonium sulfate, MPD, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG1500 | ||
| 2 | 1 | 1 | Mops-Na | ||
| 3 | 1 | 1 | ammonium sulfate | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | HOH | ||
| 6 | 1 | 2 | PEG1500 | ||
| 7 | 1 | 2 | ammonium sulfate | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | HOH |
