2CV0
Glutamyl-tRNA synthetase from Thermus thermophilus in complex with tRNA(Glu) and L-glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-04 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.727, 219.321, 135.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.960 - 2.400 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.098 | |
Number of reflections | 67216 | |
<I/σ(I)> | 14.5 | 1.92 |
Completeness [%] | 96.9 | 98.3 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | PEG1500, Mops-Na, ammonium sulfate, MPD, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG1500 | ||
2 | 1 | 1 | Mops-Na | ||
3 | 1 | 1 | ammonium sulfate | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 1 | HOH | ||
6 | 1 | 2 | PEG1500 | ||
7 | 1 | 2 | ammonium sulfate | ||
8 | 1 | 2 | MPD | ||
9 | 1 | 2 | HOH |