2CUO
Collagen model peptide (PRO-PRO-GLY)9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-20 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.946, 26.563, 80.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.330 |
| R-factor | 0.1839 |
| Rwork | 0.182 |
| R-free | 0.22870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1itt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.023 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.210 | 1.380 |
| High resolution limit [Å] | 1.330 | 1.330 |
| Rmerge | 0.070 | 0.269 |
| Number of reflections | 24336 | |
| <I/σ(I)> | 4.8 | 1.8 |
| Completeness [%] | 97.7 | 98.3 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG400, ACETIC ACID, SODIUM AZIDE, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
