2CTZ

Crystal structure of o-acetyl homoserine sulfhydrylase from Thermus thermophilus HB8

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE
Synchrotron site	SPring-8
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-11
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.9791, 0.9794, 0.9000
Spacegroup name	I 41
Unit cell lengths	149.095, 149.095, 219.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.880 - 2.600
R-factor	0.18857
Rwork	0.187
R-free	0.21716
Structure solution method	MAD
RMSD bond length	0.030
RMSD bond angle	2.517
Data reduction software	HKL-2000
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.880	2.666
High resolution limit [Å]	2.600	2.600
Rmerge	0.111	0.270
Number of reflections	69366
Completeness [%]	100.0	100
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.25% dichloromethane, 1.8M sodim/potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K