2CST

CRYSTAL STRUCTURE OF THE CLOSED FORM OF CHICKEN CYTOSOLIC ASPARTATE AMINOTRANSFERASE AT 1.9 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	56.400, 126.000, 124.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.175
RMSD bond length	0.010
RMSD bond angle	1.900
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	1.900 *
Rmerge	0.095 *
Total number of observations	310873 *
Number of reflections	69643 *
Completeness [%]	98.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	17 (%(w/v))
2	1	reservoir	sodium maleate	40 (mM)
3	1	reservoir		5 (mM)
4	1	drop	protein	10 (mg/ml)