2CSN

BINARY COMPLEX OF CASEIN KINASE-1 WITH CKI7

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Detector technology	IMAGE PLATE AREA DETECTOR
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	79.100, 79.100, 120.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.000 - 2.500
R-factor	0.178
Rwork	0.178
RMSD bond length	0.012
RMSD bond angle	23.480 *
Data reduction software	HKL ((DENZO))
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.069
Total number of observations	94790 *
Number of reflections	18581
Completeness [%]	93.5
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *	16 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	methanol	2 (%)
2	1	reservoir	ammonium sulfate	1.4-1.7 (M)
3	1	reservoir	sodium acetate	2.5-25 (mM)