2CSM

TYR-BOUND T-STATE OF YEAST CHORISMATE MUTASE

Experimental procedure

Temperature [K]	123
Collection date	1995-10-10
Detector	SIEMENS
Spacegroup name	P 43 21 2
Unit cell lengths	78.600, 78.600, 116.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.800
R-factor	0.213
Rwork	0.213
R-free	0.32100
RMSD bond length	0.007
RMSD bond angle	19.400 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Rmerge	0.069	0.280 *
Total number of observations	29418 *
Number of reflections	9401
Completeness [%]	99.1	97.3 *
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	2.1 (M)
2	1	reservoir	sodium acetate	55 (mM)
3	1	reservoir	dithiothreitol	2 (mM)
4	1	reservoir	L-tyrosine	0.7 (mM)
5	1	drop	ammonium salfate	2.1 (M)
6	1	drop	sodium acetate	55 (mM)
7	1	drop	dithiothreitol	2 (mM)
8	1	drop	L-tyrosine	0.7 (mM)
9	1	drop	YCM	15 (mg/ml)