2CRK

MUSCLE CREATINE KINASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1996-10-15
Detector	MAC Science DIP-2020
Spacegroup name	I 4 2 2
Unit cell lengths	199.600, 199.600, 71.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.350
R-factor	0.195
Rwork	0.195
R-free	0.28800
Structure solution method	MIR
Starting model (for MR)	1crk
RMSD bond length	0.014
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.101 *
Number of reflections	29042
<I/σ(I)>	16.45	3.78
Completeness [%]	96.6	89.2
Redundancy	9.4	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	standing drop *	7.2		55% TO 60% SATURATED AMMONIUM SULFATE, 2.5% PEG400 IN HEPES BUFFER AT PH 7.2, ABOUT 5MM/MG PROTEIN CONCENTRATION

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Na-HEPES	50 (mM)
3	1	reservoir	ammonium sulfate	55 (%sat)
4	1	reservoir	PEG400	2.5 (%)