2CPL

SIMILARITIES AND DIFFERENCES BETWEEN HUMAN CYCLOPHILIN A AND OTHER BETA-BARREL STRUCTURES. STRUCTURAL REFINEMENT AT 1.63 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	43.000, 52.600, 89.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 2.630 *
R-factor	0.18
Rwork	0.180
RMSD bond length	0.013
RMSD bond angle	2.500
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.630 *
Rmerge	0.042 *
Total number of observations	53638 *
Number of reflections	22219 *
Completeness [%]	91.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10-15 (mg/ml)
2	1	2	Tris-HCl	20 (mM)
3	1	2	dithiothreitol	2 (mM)
4	1	2	EDTA	2 (mM)
5	1	2		0.5 (mM)
6	1	2	PEG	10.5 (%(w/v))
7	1	2	ethanol	6.7 (%)