2CPK

CRYSTAL STRUCTURE OF THE CATALYTIC SUBUNIT OF CYCLIC ADENOSINE MONOPHOSPHATE-DEPENDENT PROTEIN KINASE

Replaces:  1CPK

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	73.620, 76.520, 80.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
Rwork	0.180
RMSD bond length	0.018
RMSD bond angle	3.600
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.040 *
Total number of observations	27067 *
Number of reflections	11291 *
Completeness [%]	87.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Bicine	50 (mM)
3	1	drop	calcium acetate	150 (mM)
4	1	drop	dithiothreitol	10 (mM)
5	1	drop	PEG400	8 (%(w/v))
6	1	reservoir	methanol	15 (%)
7	1	reservoir	dithiothreitol	10 (mM)
8	1	reservoir	PEG400	8 (%(w/v))