2COL

Crystal structure analysis of CyaA/C-Cam with Pyrophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	200
Detector technology	CCD
Collection date	2005-03-30
Detector	CUSTOM-MADE
Wavelength(s)	0.97934
Spacegroup name	P 41 21 2
Unit cell lengths	79.613, 79.613, 139.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.247
Rwork	0.240
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.023
RMSD bond angle	2.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	278	PEG4000, Na Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K