2CMP
crystal structure of the DNA binding domain of G1P SMALL TERMINASE SUBUNIT from bacteriophage SF6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 57.286, 57.286, 57.286 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.520 - 1.580 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.27600 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.488 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.400 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.040 | 0.560 |
| Number of reflections | 8787 | |
| <I/σ(I)> | 38.7 | 3.2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 9.3 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | HANGING DROP WITH 1 UL OF 17MG/ML PROTEIN IN 50MM HEPES PH7.5 10MM NACL MIXED WITH 1 UL OF 2.4 M NA MALONATE PH 7.0 AND EQUILIBRATED AGAINST 1ML OF 2.4 M NA MALONATE PH 7.0 AT 20C |
