2CMG
Crystal Structure of Spermidine Synthase from Helicobacter Pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL17B2 |
| Synchrotron site | NSRRC |
| Beamline | BL17B2 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.147, 58.141, 74.832 |
| Unit cell angles | 90.00, 90.93, 90.00 |
Refinement procedure
| Resolution | 24.830 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.27300 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 | 0.270 |
| Number of reflections | 32833 | |
| <I/σ(I)> | 19.7 | 3.4 |
| Completeness [%] | 91.9 | 87 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.50 |
