2CME
The crystal structure of SARS coronavirus ORF-9b protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 42 |
| Unit cell lengths | 140.028, 140.028, 45.146 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.800 |
| R-factor | 0.266 |
| Rwork | 0.266 |
| R-free | 0.28900 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.110 | |
| Number of reflections | 22040 | |
| <I/σ(I)> | 22.8 | 1.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 14.9 | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 32% PEG3350, 200MM MGCL2, 100MM TRIS-HCL PH8.2, pH 8.20 |
