2CM6
crystal structure of the C2B domain of rabphilin3A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 89.7 |
| Detector technology | CCD |
| Collection date | 2005-11-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.069, 59.606, 66.385 |
| Unit cell angles | 90.00, 103.89, 90.00 |
Refinement procedure
| Resolution | 43.760 - 1.850 |
| R-factor | 0.1948 |
| R-free | 0.26750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cm5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.021 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.760 | 1.940 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 25345 | |
| <I/σ(I)> | 12.5 | 3.07 |
| Completeness [%] | 97.2 | 93.9 |
| Redundancy | 6.44 | 4.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7.6, 25 % PEG 8000, 200 MM CA |
