2CM5

crystal structure of the C2B domain of rabphilin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	89.7
Collection date	2005-11-21
Wavelength(s)	0.95,0.9796
Spacegroup name	P 21 21 2
Unit cell lengths	53.898, 60.043, 41.928
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.110 - 1.280
R-factor	0.144
R-free	0.19370
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	0.030
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.110	1.380
High resolution limit [Å]	1.280	1.280
Rmerge	0.030	0.210
Number of reflections	33387
<I/σ(I)>	14.38	2.84
Completeness [%]	94.2	72.7
Redundancy	1.72	1.16

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1M HEPES PH 8.5,20 % PEG MME 2000 OR 20 % PEG 8000